Comprehensive miRNA analysis using serum from patients with non-infectious uveitis
Purpose
MicroRNAs (miRNAs) have attracted attention as a biomarker in a variety of diseases. However, extensive unbiased miRNAs analysis in patients with uveitis has not been completely explored. In the present study, we comprehensively analyzed the deregulated miRNAs in three major forms of uveitis [Behҫet's disease (BD), sarcoidosis and Vogt-Koyanagi-Harada disease (VKH)] to search for potential biomarkers.
Methods
This study included 10 BD patients, 17 sarcoidosis patients, and 13 VKH patients. 11 healthy subjects were used as controls. The miRNAs expression levels were studied by microarray using serum samples from patients with uveitis and healthy controls.
Results
A total of 281 upregulated miRNAs and 137 downregulated miRNAs were detected in BD patients, 35 upregulated miRNAs and 86 downregulated miRNAs in sarcoidosis patients, and 153 upregulated miRNAs and 35 downregulated miRNAs in VKH patients. Furthermore, we identified miR-4708-3p, miR-4323 and let-7g-3p as the best predictor miRNAs for BD, sarcoidosis and VKH, respectively. Panels of miRNAs with diagnostic potential for the three diseases were generated using machine learning.
Conclusion
In this study, comprehensive miRNA analysis identified deregulated miRNAs in three major forms of non-infectious uveitis. This study provides new insights into molecular pathogenetic mechanisms and useful information toward developing novel diagnostic biomarkers for BD, sarcoidosis, and VKH.
Conflict of interest
No
Authors 1
Last name
ASAKAGE
Initials of first name(s)
M
Department
Ophthalmology
City
Tokyo
Country
Japan
Authors 2
Last name
USUI
Initials of first name(s)
Y
Department
Ophthalmology
City
Tokyo
Country
Japan
Authors 3
Last name
NEZU
Initials of first name(s)
N
Department
Ophthalmology
City
Tokyo
Country
Japan
Authors 4
Last name
SHIMIZU
Initials of first name(s)
H
Department
Ophthalmology
City
Tokyo
Country
Japan
Authors 5
Last name
TSUBOTA
Initials of first name(s)
K
Department
Ophthalmology
City
Tokyo
Country
Japan
Authors 6
Last name
YAMAKAWA
Initials of first name(s)
N
Department
Ophthalmology
City
Tokyo
Country
Japan
Authors 7
Last name
TAKANASHI
Initials of first name(s)
M
Department
Molecular pathology
City
Tokyo
Country
Japan
Authors 8
Last name
KURODA
Initials of first name(s)
M
Department
Molecular pathology
City
Tokyo
Country
Japan
Authors 9
Last name
GOTO
Initials of first name(s)
H
Department
Ophthalmology
City
Tokyo
Country
Japan
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