| Presentation preference | Poster presentation |
| Title | Cytokines profile in ocular fluid of patients with uveitis: preliminary results |
| Purpose | To understand the pathophysiology of uveitis, we analyzed the intraocular fluid cytokine profile of individuals with uveitis. |
| Methods | Aqueous/vitreous humor samples of 35 individuals with active uveitis were analyzed: infectious uveitis (IU) [ocular toxoplasmosis (OT) (n=10), herpetic uveitis (HU) ( n=10), other infection ( n=3)] and non-infectious uveitis (NIU)(n=12). Participants undergoing senile cataract surgery were controls (n=2). Twenty cytokines, functionally classified as pro-inflammatory (INFLAMMA, n=12), immunoregulatory (REG, n=5) and repairing (REPAIR, n=3), were analyzed by multiplex microbeads immunoassay. Cytokine functional predominance based on detection rate was determined for each study group. |
| Results | Cytokines levels and detection rates did not differ among the OT, HU and NIU groups, except for IL-10 in all-group analysis (p=0.019), though not significant in the 2-by-2 analysis (HUvsOT, p=0.057). MCP-1, IP-10 and TGF-β1 were detected in all samples. IL-8 and IL-10 were detected in all uveitic samples. IL-8 levels were 2.9x higher in IU than NIU. VEGF and FGF levels were 2.1x and 1.7x higher in NIU than IU. TNF-α, IL-12p70 and IFN-γ levels were respectively 9.9, 1.5 and 14.9 folds higher in OT than HU. REG/INFLAMMA IL-4/TNF-α (2.4vs0.8, p=0.002) and REPAIR/INFLAMMA FGF-2/TNF-α (9.7vs1.3, p=0.035) level ratios were higher in HUvsOT. On cytokine functional predominance, HU had a predominant REPAIR profile vs INFLAMMA and REG cytokines, while NIU had a predominance of REPAIR vs REG cytokines. |
| Conclusion | Our preliminary results indicate that OT displays a more diverse and intense INFLAMMA cytokine profile and a potential prominent role for REG cytokines in HU and for REPAIR cytokines in NIU. |
| Conflict of interest | No |
Authors 1
| Last name | FERRACIOLI-ODA |
| Initials of first name(s) | E |
| Department | Ophthalmology, Faculdade de Medicina, Universidade de Sao Paulo |
| City | Sao Paulo |
| Country | Brazil |
Authors 2
| Last name | Coelho |
| Initials of first name(s) | V |
| Department | Laboratory of Immunology, Heart Institute, FMUSP |
| City | Sao Paulo |
| Country | Brazil |
Authors 3
| Last name | Tanaka |
| Initials of first name(s) | T |
| Department | Ophthalmology, Faculdade de Medicina, Universidade de Sao Paulo |
| City | Sao Paulo |
| Country | Brazil |
Authors 4
| Last name | Gouveia |
| Initials of first name(s) | MGS |
| Department | Clinical and Experimental Gastroenterology Laboratory, LIM 07, FMUSP |
| City | Sao Paulo |
| Country | Brazil |
Authors 5
| Last name | Pinho |
| Initials of first name(s) | JR |
| Department | Clinical and Experimental Gastroenterology Laboratory, LIM 07, FMUSP |
| City | Sao Paulo |
| Country | Brazil |
Authors 6
| Last name | Bispo |
| Initials of first name(s) | PJ |
| Department | Ophthalmology, Harvard Medical School |
| City | Boston |
| Country | United States |
Authors 7
| Last name | Hirata |
| Initials of first name(s) | CE |
| Department | Ophthalmology, Faculdade de Medicina, Universidade de Sao Paulo |
| City | Sao Paulo |
| Country | Brazil |
Authors 8
| Last name | Antonangelo |
| Initials of first name(s) | L |
| Department | Laboratorial Medicine Laboratory, LIM 03, FMUSP |
| City | Sao Paulo |
| Country | Brazil |
Authors 9
| Last name | Yamamoto |
| Initials of first name(s) | JH |
| Department | Ophthalmology, LIM 33, Faculdade de Medicina, Universidade de Sao Paulo |
| City | Sao Paulo |
| Country | Brazil |
