| Presentation preference | Oral presentation |
| Title | Cytokines, chemokines and growth factors profile in human aqueous humor in idiopathic uveitis |
| Purpose | To investigate which cytokines, chemokines, growth factors are involved in the immunopathogenesis of idiopathic uveitis.
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| Methods | Serum and aqueous humor (AH) samples of 75 patients with idiopathic uveitis were analyzed by multiplex immunoassay. Infectious controls consisted of 16 patients with ocular toxoplasmosis. Noninfectious controls consisted of 7 patients with Behçet disease uveitis and 15 patients with sarcoidosis uveitis. In each sample, 27 immune mediators ± IL-21 and IL-23 were measured. |
| Results | In idiopathic uveitis, 13 of the 29 mediators, including most proinflammatory and vascular mediators such as IL-6, IL-8, IL-12, G-CSF, GM-CSF, MCP-1, IP-10, TNF-α and VEGF, were significantly elevated in the AH when compared to all controls. We clustered 4 subgroups of idiopathic uveitis (statistical analysis of hierarchical unsupervised classification), characterized by the order of magnitude of concentrations of intraocular cytokines. We associated these 4 subgroups to their demographics, and clinical characteristics. Group1 had relatively weak cytokine values with mostly posterior uveitis and retinal vasculitis; group2 presented moderately high cytokine values. In that group intermediate uveitis was more frequent with macular edema; group3 presented moderate high cytokine values (IL-6, TNF-α, IP-10) except for the IL-12, which was very high. In that group, posterior uveitis was more frequent; and finally, group4 had very high cytokines and chemokines values. In that group, posterior and panuveitis were more frequent (38%, each) and with a macular edema (42%). |
| Conclusion | This study suggests various clusters among the idiopathic uveitis group rather than one specific uveitis entity. |
| Conflict of interest | No |
Authors 1
| Last name | ERRERA |
| Initials of first name(s) | MH |
| Department | UPMC Eye Center, University of Pittsburgh School of Medicine, Pennsylvania |
| City | Pittsburgh |
| Country | United States |
Authors 2
| Last name | Pratas |
| Initials of first name(s) | A |
| Department | Laboratory at Quinze-Vingts National Eye Hospital |
| City | Paris |
| Country | France |
Authors 3
| Last name | Fisson |
| Initials of first name(s) | S |
| Department | Généthon, Inserm UMR_S951, Univ Evry, Université Paris-Saclay, EPHE |
| City | Evry |
| Country | France |
Authors 4
| Last name | Manicom |
| Initials of first name(s) | T |
| Department | Laboratory at Quinze-Vingts National Eye Hospital |
| City | Paris |
| Country | France |
Authors 5
| Last name | Boubaya |
| Initials of first name(s) | M |
| Department | Hôpital Avicenne, URC-CRC GHPSS |
| City | Bobigny |
| Country | France |
Authors 6
| Last name | Sahel |
| Initials of first name(s) | JA |
| Department | Departments of Ophthalmology at Quinze-Vingts National Eye Hospital and DHU Si |
| City | Paris |
| Country | France |
Authors 7
| Last name | Goldschmidt |
| Initials of first name(s) | P |
| Department | Laboratory at Quinze-Vingts National Eye Hospital |
| City | Paris |
| Country | France |
Authors 8
| Last name | Brignole-Baudouin |
| Initials of first name(s) | F |
| Department | Laboratory at Quinze-Vingts National Eye Hospital; Faculty Pharmacy, Sorbonne Universities |
| City | Paris |
| Country | France |
Authors 9
| Last name | Bodaghi |
| Initials of first name(s) | B |
| Department | Pitié-Salpêtrière Hospital, DHU Sight Restore |
| City | Paris |
| Country | France |
Authors 10
| Last name | Bloch-Queyrat |
| Initials of first name(s) | C |
| Department | Hôpital Avicenne, URC-CRC GHPSS |
| City | Bobigny |
| Country | France |
