| Presentation preference | Oral presentation |
| Title | Inflammatory molecules in aqueous humor of children with endogenous uveitis |
| Accept poster if oral is not possible ? | Yes |
| Purpose | To investigate immune mediator profile in the aqueous humor (AqH) of children with endogenous uveitis (EU) and its relationships with disease location, activity and presence of secondary glaucoma. |
| Methods | Concentrations of 45 inflammatory molecules were measured using multiplex bead immunoassay (xMAP, Luminex, USA; cytokine/chemokine/growth factor 45-plex human panel, ProcartaPlex, eBioscience, Austria) and TGF-β1, TGF-β2 using enzyme-linked immunosorbent assay (ELISA) (Bender Medsystems, Austria) in 43 AqH samples obtained during cataract surgery from 40 children aged 5 – 15 years with anterior (32 eyes), intermediate (8), posterior (1) or panuveitis (2). Remission of uveitis was in 27 eyes, mild activity in 16. |
| Results | Frequency of detection of molecules varied and was highest (70 – 100%) for MCP-1, MIP-1α, MIP-1β, Eotaxin, IP-10, VEGF-A, TGF-β1, TGF-β2, SDF-1α, HGF, SCF, IL-7, IL-6 and LIF. Levels of IL-2 (p=0.038), Eotaxin (p=0.02), TGF-beta2 (p=0.008) were higher in anterior than in intermediate uveitis. Levels of IL-10 (p=0.03) were higher, IL-2 (p=0.017) and EGF (p=0,003) lower in remission than in active uveitis. Higher levels of IL-7 (p=0.017) and tendency to higher levels MCP-1 (p=0.064) and lower levels Eotaxin (p= 0,059) and IL-15 (p=0,075) were found in patients with than in patients without uveitic glaucoma. |
| Conclusion | Multiple inflammatory molecules, detected in AqH of children with EU, may play pathogenic role in the disease and serve as targets for development of treatment. |
| Conflict of interest | No |
Author 1
| Last name | DENISOVA |
| Initials of first name(s) | E. |
| Department | Helmholtz Research Institute of Eye Diseases, Moscow, Russia |
| City | Moscow |
| Country | Russian Federation |
Author 2
| Last name | Balatskaya |
| Initials of first name(s) | N. |
| City | Moscow |
| Country | Russian Federation |
Author 3
| Last name | Kulikova |
| Initials of first name(s) | I. |
| City | Moscow |
| Country | Russian Federation |
Author 4
| Last name | Katargina |
| Initials of first name(s) | L. |
| City | Moscow |
| Country | Russian Federation |
Author 5
| Last name | Ibaid |
| Initials of first name(s) | B.N. |
| City | Moscow |
| Country | Russian Federation |
