| Presentation preference | Oral presentation |
| Title | Histopathology, immunohistochemistry and molecular biologic study of eviscerated / enucleated specimen of end-stage ocular inflammatory diseases. |
| Purpose | To describe the histopathology (HPE), immunohistochemistry (IHC) and molecular biologic study of several end stage ocular inflammatory diseases |
| Methods | HPE, IHC and molecular biologic study of such eyes of 18 eviscerated and enucleated globes secondary to the intractable intraocular inflammation was done. |
| Results | Among cases of tubercular panuveitis, in one eye of subretinal abscess had acid-fast bacilli in the subretinal space. In other three cases, M.TB DNA was detected polymerase chain reaction (PCR). In a case of tuberculous sclerouveitis, HPE showed chronic granulomatous inflammation of the sclera. PCR of the paraffin section was positive for IS6110 gene of M.TB. In a case of end-stage pars planitis, HPE showed acellular fibrous tissue in the peripheral retina with chronic granulomatous inflammation. IHC showed CD3 and CD20 positivity. In a case of Eales’ disease, HPE showed multiple vascular channels surrounded by lymphocytes. IHC showed CD3, CD 8 and CD 68 positivity of the cellular infiltrates. In a case of acute retinal necrosis, chronic non-granulomatous inflammation of the retina with occluded retinal blood vessels was seen. Herpes virus inclusion bodies were seen on HPE. IHC showed CD 3 positivity. PCR was positive for varicella zoster virus DNA. In a case of VKH, predominantly CD 20 positive lymphocytic infiltration in the choroid was seen. In sympathetic ophthalmia, predominantly nongranulomatous inflammation with CD 3 positivity was seen. |
| Conclusion | Histopathology, microbiology, Immunohistochemistry and molecular biologic study can provide valuable insight regarding the diagnosis and pathogenesis of several ocular inflammatory diseases. |
| Conflict of interest | No |
Authors 1
| Last name | BISWAS |
| Initials of first name(s) | J |
| Department | Uveitis and ocular pathology |
| City | Chennai |
| Country | India |
Authors 2
| Last name | Patnaik |
| Initials of first name(s) | G |
| Department | Uveitis |
| City | Kolkata |
| Country | India |
