Identification and characterization of dendritic cells in glaucomatous eyes: A promising futuristic target for precision anti-glaucoma drug delivery
Purpose
To present identification and characterization of dendritic cells in glaucomatous eyes
Methods
Observational study in post enucleated and post mortem glaucomatous eye specimens Analysis of enucleated eyeballs’ optic nerve in a gross pathology, stained specimen and immunohistochemistry (IHC) that were carried out to know the anatomical pigmented cells arrangements in an around the edges of the optic disc. Total eight enucleated specimens from seven patients for various other indications were studied including two postmortem eyes from a patient which were taken after proper consent. Routine stains with H&E, diffractive photography, Trabecular meshwork (TM) and retinal ganglion cells (RGCs) of these cases were studied for the association.
Results
Five cases had some clue of glaucomatous change. Two eyes of one patient was enucleated after death(postmortem) with proper eye-bank consent for research who had 30 years of glaucoma; One, post trabeculectomized eye with history of trauma and painful blind eye, two other cases removed for painful blind eyes with raised IOPs which include one phthisical eye. Other three eyes were of different indications removed in OT after pre-operative consent. Pigmented cells from the uveal tissues were spotted near the peripheral edges of the optic nerve. Their morphological sizes, shape, direction of advancement were documented. In all these cases, the TM and RGCs were studied for the similar pigmented cells for the correlation. IHC with control were done for BAX, BCL2 and CD 68 for peripapillary cells, TM and RGCs.
Conclusion
The study evaluated the presence of dendritic cells in the targeted sites of glaucoma