Dietary intake of whale oil containing ω-3 long-chain polyunsaturated fatty acids attenuates choroidal neovascularization in mice
Accept poster if oral is not possible ?
Yes
Purpose
Since anti-vascular endothelial growth factor (VEGF) therapy is available for the regression of choroidal neovascularization (CNV), it does not work for the pathophysiology of Age-related macular degeneration (AMD), so a cure is increasingly demanded. Whale oil promotes various bodily functions, such as anti-inflammatory effects for cardiovascular disease, but its physiological mechanisms are still unclarified. Here, we examined the effects of whale oil on a mouse model of AMD.
Methods
C57BL/6 mice were given to a diet that was either supplemented with whale oil or free of ω-3 long-chain polyunsaturated fatty acids (LCPUFAs). Mice were subjected to laser photocoagulation 2 weeks after the diet respectively.
Results
The area of CNV measured in choroidal flat-mount preparations at 7 days after laser photocoagulation was significantly smaller in mice fed whale oil compared with control mice free of ω-3 LCPUFAs. The plasma concentrations of ω-3 LCPUFAs were higher, whereas those of ω-6 LCPUFAs were lower in mice fed the diet containing whale oil than in those fed the control diet. The concentrations of various inflammatory cytokines and chemokines in the retina or choroid at 3 or 7 days after CNV induction differed between the two groups of mice. The concentration of VEGF was decreased in the retina but increased in the choroid at 7 or 3 days after photocoagulation, respectively.
Conclusion
Dietary intake of whale oil containing ω-3 LCPUFAs attenuated CNV in association with changes in inflammatory mediator levels and VEGF expression in the retina and choroid of mice, and it, therefore, warrants further study as a means to protect against AMD in humans.
Conflict of interest
No
1
Last name
YANAI
Initials of first name(s)
R
Department
Ophthalmology
City
Tokushima
Country
Japan
2
Last name
Yasunaga
Initials of first name(s)
G
Department
Institute of Cetacean Research
City
Chuo-ku
Country
Japan
3
Last name
Tsuji
Initials of first name(s)
S
Department
Pharmacology
City
Ube
Country
Japan
4
Last name
Honda
Initials of first name(s)
T
Department
Pharmacology
City
Ube
Country
Japan
5
Last name
Iwata
Initials of first name(s)
A
Department
Yamaguchi Prefectural Industrial Technology Institute
City
Ube
Country
Japan
6
Last name
Miyagawa
Initials of first name(s)
e
Department
Yamaguchi Prefectural Industrial Technology Institute
City
Ube
Country
Japan
7
Last name
Kishimoto
Initials of first name(s)
M
Department
Faculty of Economics
City
Shimonoseki
Country
Japan
8
Last name
Sakai
Initials of first name(s)
H
Department
Institute of Cetacean Research
City
Chuo-ku
Country
Japan
9
Last name
Fujise
Initials of first name(s)
Y
Department
Institute of Cetacean Research
City
Chuo-ku
Country
Japan
10
Last name
Asagiri
Initials of first name(s)
M
Department
Pharmacology
City
Ube
Country
Japan
11
Last name
Mitamura
Initials of first name(s)
Y
Department
Ophthalmology
City
Tokushima
Country
Japan
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