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Title	Comprehensive miRNA analysis using serum from patients with non-infectious uveitis

Purpose	MicroRNAs (miRNAs) have attracted attention as a biomarker in a variety of diseases. However, extensive unbiased miRNAs analysis in patients with uveitis has not been completely explored. In the present study, we comprehensively analyzed the deregulated miRNAs in three major forms of uveitis [Behҫet's disease (BD), sarcoidosis and Vogt-Koyanagi-Harada disease (VKH)] to search for potential biomarkers.
Methods	This study included 10 BD patients, 17 sarcoidosis patients, and 13 VKH patients. 11 healthy subjects were used as controls. The miRNAs expression levels were studied by microarray using serum samples from patients with uveitis and healthy controls.
Results	A total of 281 upregulated miRNAs and 137 downregulated miRNAs were detected in BD patients, 35 upregulated miRNAs and 86 downregulated miRNAs in sarcoidosis patients, and 153 upregulated miRNAs and 35 downregulated miRNAs in VKH patients. Furthermore, we identified miR-4708-3p, miR-4323 and let-7g-3p as the best predictor miRNAs for BD, sarcoidosis and VKH, respectively. Panels of miRNAs with diagnostic potential for the three diseases were generated using machine learning.
Conclusion	In this study, comprehensive miRNA analysis identified deregulated miRNAs in three major forms of non-infectious uveitis. This study provides new insights into molecular pathogenetic mechanisms and useful information toward developing novel diagnostic biomarkers for BD, sarcoidosis, and VKH.
Conflict of interest	No

Authors 1

Last name	ASAKAGE
Initials of first name(s)	M
Department	Ophthalmology
City	Tokyo
Country	Japan

Authors 2

Last name	USUI
Initials of first name(s)	Y
Department	Ophthalmology
City	Tokyo
Country	Japan

Authors 3

Last name	NEZU
Initials of first name(s)	N
Department	Ophthalmology
City	Tokyo
Country	Japan

Authors 4

Last name	SHIMIZU
Initials of first name(s)	H
Department	Ophthalmology
City	Tokyo
Country	Japan

Authors 5

Last name	TSUBOTA
Initials of first name(s)	K
Department	Ophthalmology
City	Tokyo
Country	Japan

Authors 6

Last name	YAMAKAWA
Initials of first name(s)	N
Department	Ophthalmology
City	Tokyo
Country	Japan

Authors 7

Last name	TAKANASHI
Initials of first name(s)	M
Department	Molecular pathology
City	Tokyo
Country	Japan

Authors 8

Last name	KURODA
Initials of first name(s)	M
Department	Molecular pathology
City	Tokyo
Country	Japan

Authors 9

Last name	GOTO
Initials of first name(s)	H
Department	Ophthalmology
City	Tokyo
Country	Japan