This abstract is assigned to sessions
| Presentation preference | Poster presentation |
| Title | Biomarker concentrations in serum and urine of ocular tuberculosis patients |
| Purpose | Ocular tuberculosis (OTB) is difficult to diagnose and alternative biofluids are being investigated to search for biomarker (BM) profiles to aid diagnosis. We questioned whether differences exist in BM concentrations in serum and urine of OTB patients.
|
| Methods | Serum and urine were collected from 14 patients with OTB. The concentrations of 29 BM were measured using the Luminex® platform. BM were selected based on earlier publications. |
| Results | The median age (7 males) was 38.5 years (range 25 – 73) with 3 cases bilateral. There were 5 cases each of posterior and panuveitis and 4 of anterior uveitis. Only 3 BM (sIL-2Ra, sTNFRI and IFNγ) showed no difference in concentration between urine and serum. Two others (sTNFRII and VEGF-A) trended towards a difference while the remaining 24 BM showed marked differences (p<0.01). Of these, only 2 (IL-1RA and IL-2) showed higher urine concentrations. |
| Conclusion | The concentrations of most BM studied differed markedly in urine and serum with the majority being much lower in urine. In most cases it is impossible to extrapolate urine concentrations from serum values and vice versa and thus different biofluids are not interchangeable when developing OTB biomarkers. |
| Conflict of interest | No |
Authors 1
| Last name | SMIT |
| Initials of first name(s) | DP |
| Department | Ophthalmology, Stellenbosch University |
| City | Cape Town |
| Country | South Africa |
Authors 2
| Last name | Van der Colff |
| Initials of first name(s) | F |
| Department | Ophthalmology, Stellenbosch University |
| City | Cape Town |
| Country | South Africa |
Authors 3
| Last name | Chegou |
| Initials of first name(s) | NN |
| Department | Molecular Biology, Stellenbosch University |
| City | Cape Town |
| Country | South Africa |
Authors 4
| Last name | Snyders |
| Initials of first name(s) | C |
| Department | Molecular Biology, Stellenbosch University |
| City | Cape Town |
| Country | South Africa |
Authors 5
| Last name | Walzl |
| Initials of first name(s) | G |
| Department | Molecular Biology, Stellenbosch University |
| City | Cape Town |
| Country | South Korea |
