This abstract is assigned to sessions
| Presentation preference | Poster presentation |
| Title | Detection of Rubella Virus (RV) in Fuchs Uveitis Syndrome (FUS) |
| Purpose | To report the presence of RV in aqueous humor (AH) in patients (PTs) with FUS |
| Methods | The results of consecutive slit lamp performed AH taps obtained between March 2018 - June 2021, in PTs diagnosed of FUS, who presented chronic intermedial uveitis or iridocyclitis, diffuse iris atrophy ± heterochromia, KPs scattered over corneal endothelium, cataract or pseudophakia, absence of posterior synechia and macular edema, were retrospectively recorded. In every eye, AH analysis included: RV Goldmann-Witmer coefficient (GWC), positive (+) if >3, and simultaneously PCR multiplex for: CMV, HSV1-2, VZV, HHV6-7-8 and EVB. In selected cases PCR for RV and GWCs for CMV, HSV1-2, VZV, or Toxoplasma, were also recorded. Except for an in-house RV PCR-real time technic, commercially available kits were used. The number of taps and related complications were recorded |
| Results | Twenty eyes of 18 PTs were included, in 15 (83,3%) RV was detected, in 14 (77,7%) by GWC (16 eyes), in 1/14 (7,1%) (15 eyes) by PCR, and in 4/14 (28,5%) by both. CMV was not detected in any eye by PCR or by GWC (13) analysis. PCR detected EBV and HHV6 in 1 eye (+) for RV (PCR / GWC). In 5 PTs/eyes only 1 negative (-) for RV, GWCs were (-) for VZV (1), HSV (3) and Toxoplasma (1). Two of the 3 (-) RV GWC patients had also (-) results for RV PCR and GWCs HSV. After Two taps per eye, hyphema occurred in 7 (35%), one of them required vitrectomy |
| Conclusion | RV is the main etiology of SUF in our PTs, the detection of viral antibodies in AH is more accurate |
| Conflict of interest | No |
Authors 1
| Last name | GONZÁLEZ-GUIJARRO |
| Initials of first name(s) | J |
| Department | Opthalmology. Hospital Universitario de la Princesa |
| City | Madrid |
| Country | Spain |
Authors 2
| Last name | BELMONTE GRAU |
| Initials of first name(s) | M |
| Department | Ophtalmology |
| City | Madrid |
| Country | Spain |
Authors 3
| Last name | MARTINEZ FLETA |
| Initials of first name(s) | P |
| Department | Immunology |
| City | Madrid |
| Country | Spain |
Authors 4
| Last name | Zurita Cruz |
| Initials of first name(s) | N |
| Department | Microbiology |
| City | Madrid |
| Country | Spain |
