Nl-Fr

View abstract

This abstract is assigned to session 21 - Free Papers IV - Potpourri
Presentation preference Oral presentation
TitleA Transcriptional-Network in Blood Dendritic Cells Links a Cytokine-Secreting CD36+CX3CR1+CD1C+ subset to Autoimmunity in the Eye
PurposeCD1c+ conventional dendritic cells (CD1c+ DCs) are increased in blood of noninfectious uveitis patients
MethodsRNA-sequencing of CD1c+ dendritic cells in two cohorts of in total 80 patients and controls was conducted.
ResultsThe CD1c+ DCs pool from patients with autoimmune uveitis (n=45) is skewed towards a transcriptional network characterized by surface receptor genes CX3CR1, CCR2, and CD36. We confirmed the association of the transcriptional network with autoimmune uveitis in another case-control cohort (n=35) and demonstrated that it was governed by NOTCH2-RUNX3 signaling. Unbiased flow cytometry analysis based on the transcriptional network identified blood CD1c+ DC subsets that can be distinguished by CX3CR1 and CD36 surface expression. A CD36+CX3CR1+CD1c+ DC subset was diminished in peripheral blood of patients and CD1c+ DCs expressing CD36 and CX3CR1 accumulate locally. The CD36+CX3CR1+CD1c+ DC subset showed a differential capacity to produce cytokines, including TNF-alpha, IL-6, and VEGF, but not IL-23.
ConclusionCollectively, these results show that CD1c+ DC subsets defined on the basis of surface expression of CD36 and CX3CR1 are linked to human autoimmune uveitis and show a differential capacity to secrete proinflammatory mediators that participate in its pathophysiology.
Conflict of interestNo
Authors 1
Last nameKUIPER
Initials of first name(s)JJW
DepartmentOphthalmology/CTI
CityUtrecht
CountryNetherlands
Authors 2
Last nameHiddingh
Initials of first name(s)S
DepartmentCTI/Ophthalmology
CityUtrecht
CountryNetherlands
Authors 3
Last namePandit
Initials of first name(s)A
DepartmentCTI/DIGD
CityUtrecht
CountryNetherlands
Authors 4
Last nameVerhagen
Initials of first name(s)FH
DepartmentOphthalmology
CityUtrecht
CountryNetherlands
Authors 5
Last nameRijken
Initials of first name(s)R
DepartmentCTI/DIGD
CityUtrecht
CountryNetherlands
Authors 6
Last nameServaas
Initials of first name(s)NH
DepartmentCTI
CityUtrecht
CountryNetherlands
Authors 7
Last nameWichers
Initials of first name(s)CGK
DepartmentCTI/DIGD
CityUtrecht
CountryNetherlands
Authors 8
Last nameten Dam-van Loon
Initials of first name(s)NH
DepartmentOphthalmology
CityUtrecht
CountryNetherlands
Authors 9
Last nameRadstake
Initials of first name(s)TRDJ
DepartmentDIGD
CityUtrecht
CountryNetherlands
Authors 10
Last namede Boer
Initials of first name(s)JH
DepartmentOphthalmology
CityUtrecht
CountryNetherlands